• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CEA/CD66e Recombinant antibody

CEA/CD66e Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240666B12

Cat no : 83653-5-RR

Print datasheet

Synonyms

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

验证数据展示

  • WB analysis of MCF-7 using 83653-5-RR

    WB analysis of MCF-7 using 83653-5-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83653-5-RR

    IHC staining of human ovary cancer using 83653-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human ovary cancer using 83653-5-RR

    IHC staining of human ovary cancer using 83653-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IF Staining of HT-29 using 83653-5-RR

    IF Staining of HT-29 using 83653-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83653-5-RR

    Affinity and Kinetic Characterization of 83653-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, human saliva
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83653-5-RR is CEA/CD66e Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Synonyms CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
Calculated Molecular Weight 77 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_004363
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06731-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

实验方案

Product Specific Protocols
WB protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
IHC protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
IF protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols