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  • Featured Product
  • KD/KO Validated

CEA/CD66e Polyclonal antibody, PBS Only

CEA/CD66e Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10421-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 10421-1-AP (same clone as 10421-1-PBS)

    WB analysis using 10421-1-AP (same clone as 10421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

产品信息

10421-1-PBS targets CEA/CD66e in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CEA/CD66e fusion protein Ag0672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Synonyms
Calculated Molecular Weight 77 kDa
Observed Molecular Weight180-200 kDa, 77 kDa
GenBank Accession NumberBC034671
Gene Symbol CEA
Gene ID (NCBI) 1048
RRIDAB_2244691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.