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验证数据展示

  • IHC staining of human colon cancer using 31519-1-AP

    IHC staining of human colon cancer using 31519-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31519-1-AP (CEA/CD66e antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human colon cancer using 31519-1-AP

    IHC staining of human colon cancer using 31519-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31519-1-AP (CEA/CD66e antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included

CEA/CD66e Polyclonal antibody

CEA/CD66e Polyclonal Antibody for IHC, ELISA
Cat No. 31519-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CEA, Meconium antigen 100, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31519-1-AP targets CEA/CD66e in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Synonyms CEA, Meconium antigen 100, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5
Calculated Molecular Weight 77 kDa
GenBank Accession NumberNM_004363
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP06731-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

实验方案

Product Specific Protocols
IHC protocol for CEA/CD66e antibody 31519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols