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验证数据展示

  • WB analysis of HeLa using 17209-1-AP

    WB analysis of HeLa using 17209-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17209-1-AP

    IF Staining of HeLa using 17209-1-AP

    Immunofluorescent analysis of Hela cells, using CEACAM21 antibody 17209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CEACAM21 Polyclonal antibody

CEACAM21 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17209-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

CEACAM21, CEACAM3, R29124_1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CEACAM21 fusion protein Ag10992 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 21
别名 CEACAM21, CEACAM3, R29124_1
计算分子量191aa,22 kDa; 293aa,32 kDa
观测分子量 32 kDa, 22 kDa
GenBank蛋白编号BC012001
基因名称 CEACAM21
Gene ID (NCBI) 90273
RRIDAB_2077481
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KPI0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CEACAM21 antibody 17209-1-APDownload protocol
IF protocol for CEACAM21 antibody 17209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols