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Conjugate
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Conjugate
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CEACAM21 Polyclonal antibody

CEACAM21 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17209-1-AP

Print datasheet

Synonyms

CEACAM21, CEACAM3, R29124_1

验证数据展示

  • WB analysis of HeLa using 17209-1-AP

    WB analysis of HeLa using 17209-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17209-1-AP (CEACAM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17209-1-AP

    IF Staining of HeLa using 17209-1-AP

    Immunofluorescent analysis of Hela cells, using CEACAM21 antibody 17209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17209-1-AP targets CEACAM21 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CEACAM21 fusion protein Ag10992 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 21
Synonyms CEACAM21, CEACAM3, R29124_1
Calculated Molecular Weight191aa,22 kDa; 293aa,32 kDa
Observed Molecular Weight 32 kDa, 22 kDa
GenBank Accession NumberBC012001
Gene Symbol CEACAM21
Gene ID (NCBI) 90273
RRIDAB_2077481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KPI0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CEACAM21 antibody 17209-1-APDownload protocol
IF protocol for CEACAM21 antibody 17209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols