验证数据展示
经过测试的应用
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| IF | See 1 publications below |
产品信息
19496-1-AP targets CEACAM3-Specific in IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | carcinoembryonic antigen-related cell adhesion molecule 3 |
| 别名 | CEACAM3, W264, CGM1, CD66D, Carcinoembryonic antigen-related cell adhesion molecule 3 |
| 计算分子量 | 27 kDa |
| GenBank蛋白编号 | NM_001815 |
| 基因名称 | CEACAM3 |
| Gene ID (NCBI) | 1084 |
| RRID | AB_2878582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40198 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEACAM3, also named as CD66D and CGM1, belongs to the immunoglobulin superfamily and CEA family. CEACAM3 is the major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species. CEACAM3 plays an important role in the clearance of pathogens by the innate immune system. It is responsible for RAC1 stimulation in the course of pathogen phagocytosis. The antibody is specific to isoform1 of CEACAM3.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CEACAM3-Specific antibody 19496-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma | ||
Cell Biosci Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis. |
