CEBPZ Polyclonal antibody

CEBPZ Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25612-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

CBF, CBF2, CCAAT binding factor, CEBPZ, HSP CBF, NOC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25612-1-AP targets CEBPZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CEBPZ fusion protein Ag11003 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCAAT/enhancer binding protein (C/EBP), zeta
别名 CBF, CBF2, CCAAT binding factor, CEBPZ, HSP CBF, NOC1
计算分子量 1054 aa, 121 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC034475
基因名称 CEBPZ
Gene ID (NCBI) 10153
RRIDAB_2880160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03701
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.

实验方案

Product Specific Protocols
WB protocol for CEBPZ antibody 25612-1-APDownload protocol
IF protocol for CEBPZ antibody 25612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Sci

CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation.

Authors - Hanxiao Shi
mouseWB

Mol Cell Proteomics

Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.

Authors - Tong Wu
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