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CELSR3 Polyclonal antibody

CELSR3 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28835-1-AP

Print datasheet

Synonyms

Cadherin family member 11, CDHF11, CELSR3, EGF like protein 1, EGFL1, Flamingo homolog 1, FMI1, HFMI1, KIAA0812, MEGF2, RESDA1

验证数据展示

  • IF Staining of mouse cerebellum using 28835-1-AP

    IF Staining of mouse cerebellum using 28835-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CELSR3 antibody (28835-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse cerebellum using 28835-1-AP

    IHC staining of mouse cerebellum using 28835-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28835-1-AP (CELSR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28835-1-AP targets CELSR3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen CELSR3 fusion protein Ag28746 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)
Synonyms Cadherin family member 11, CDHF11, CELSR3, EGF like protein 1, EGFL1, Flamingo homolog 1, FMI1, HFMI1, KIAA0812, MEGF2, RESDA1
Calculated Molecular Weight 358 kDa
GenBank Accession NumberNM_001407
Gene Symbol CELSR3
Gene ID (NCBI) 1951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Atypical cadherin Celsr3 plays critical roles during brain development, in collaboration with Fzd3. Celsr3 is required for postnatal cerebellar Purkinje cell maturation and synaptic plasticity, which has potential implications in neural disorders implicating the cerebellum.

实验方案

Product Specific Protocols
IHC protocol for CELSR3 antibody 28835-1-APDownload protocol
IF protocol for CELSR3 antibody 28835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols