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验证数据展示

  • IF Staining of mouse cerebellum using 28835-1-AP

    IF Staining of mouse cerebellum using 28835-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CELSR3 antibody (28835-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse cerebellum using 28835-1-AP

    IHC staining of mouse cerebellum using 28835-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28835-1-AP (CELSR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CELSR3 Polyclonal antibody

CELSR3 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 28835-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

IHC, IF-P, ELISA

别名

Cadherin family member 11, CDHF11, CELSR3, EGF like protein 1, EGFL1, Flamingo homolog 1, FMI1, HFMI1, KIAA0812, MEGF2, RESDA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28835-1-AP targets CELSR3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 CELSR3 fusion protein Ag28746 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)
别名 Cadherin family member 11, CDHF11, CELSR3, EGF like protein 1, EGFL1, Flamingo homolog 1, FMI1, HFMI1, KIAA0812, MEGF2, RESDA1
计算分子量 358 kDa
GenBank蛋白编号NM_001407
基因名称 CELSR3
Gene ID (NCBI) 1951
RRIDAB_3086089
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYQ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Atypical cadherin Celsr3 plays critical roles during brain development, in collaboration with Fzd3. Celsr3 is required for postnatal cerebellar Purkinje cell maturation and synaptic plasticity, which has potential implications in neural disorders implicating the cerebellum.

实验方案

Product Specific Protocols
IHC protocol for CELSR3 antibody 28835-1-APDownload protocol
IF protocol for CELSR3 antibody 28835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols