验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
12841-1-AP targets CENPH in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CENPH fusion protein Ag3836 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | centromere protein H |
| Synonyms | CENP H, CENPH, centromere protein H, ICEN35, NNF1, PMF1 |
| Calculated Molecular Weight | 247 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC015355 |
| Gene Symbol | CENPH |
| Gene ID (NCBI) | 64946 |
| RRID | AB_2076634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3R5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Centromere protein H (CENPH) is a component of the active centromere complex that associates with centromeric DNA and binds spindle microtubules to the chromosomes, which is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPH plays a key role in assembly of kinetochore proteins, mitotic progression and chromosome segregation that may cause misaligned chromosomes and multipolar spindles. Besides, CENPH may be associated with the modulation of the cell cycle through an interaction with CENPC. It has been shown that the expression of CENPH is related to clinical application including colorectal cancer, gastric cancer, renal cell carcinoma, hepatocellular carcinoma and other poor prognosis in various solid tumors. (PMID: 26378239, 27993978, 28819418)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPH antibody 12841-1-AP | Download protocol |
| IP protocol for CENPH antibody 12841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Cancer Res CENPH overexpression promotes the progression, cisplatin resistance, and poor prognosis of lung adenocarcinoma via the AKT and ERK/P38 pathways
|