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- KD/KO Validated
CENPK Polyclonal antibody
CENPK Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
The immunogen of 26208-1-AP is CENPK Fusion Protein expressed in E. coli.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | CENPK fusion protein Ag23823 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | centromere protein K |
| Synonyms | AF5alpha, CENP K, CENPK, centromere protein K, FKSG14, ICEN37, P33, Protein AF 5alpha, Solt |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC005400 |
| Gene Symbol | CENPK |
| Gene ID (NCBI) | 64105 |
| RRID | AB_2880427 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BS16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPK antibody 26208-1-AP | Download protocol |
| IP protocol for CENPK antibody 26208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol m6A demethylase ALKBH5 maintains stemness of intrahepatic cholangiocarcinoma by sustaining BUB1B expression and cell proliferation | ||
Cancer Cell Int Bioinformatics analysis combined with experiments predicts CENPK as a potential prognostic factor for lung adenocarcinoma.
| ||
Toxicol Appl Pharmacol The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway
| ||
Bioengineered Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration
| ||
Onco Targets Ther Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1. |