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CENPM Polyclonal antibody

CENPM Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19840-1-AP

Print datasheet

Synonyms

ICEN39, Centromere protein M, CENP-M, CENP M, C22orf18

验证数据展示

  • WB analysis using 19840-1-AP

    WB analysis using 19840-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19840-1-AP (CENPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 19840-1-AP

    IF Staining of NIH/3T3 using 19840-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Raji cells
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19840-1-AP targets CENPM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CENPM fusion protein Ag13843 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centromere protein M
Synonyms ICEN39, Centromere protein M, CENP-M, CENP M, C22orf18
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC000705
Gene Symbol CENPM
Gene ID (NCBI) 79019
RRIDAB_3661902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSP4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Centromere protein M (CENPM), also referred as proliferation associated nuclear element 1 (PANE1), is one of the components of a complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The expression of CENPM and centromere assembly should be controlled closely during the cell cycle, and mistakes in this regulation may result in aneuploidy (PMID: 32705259). CENPM has been reported as a novel biomarker of hepatocellular carcinoma, melanoma and bladder cancer (PMID: 31703591).

实验方案

Product Specific Protocols
WB protocol for CENPM antibody 19840-1-APDownload protocol
IF protocol for CENPM antibody 19840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols