验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
16751-1-AP targets CENPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | CENPN fusion protein Ag10219 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centromere protein N |
| 别名 | BM039, C16orf60, CENP N, CENPN, centromere protein N, ICEN32 |
| 计算分子量 | 339 aa, 40 kDa |
| 观测分子量 | 40 kDa, 25-30 kDa |
| GenBank蛋白编号 | BC008972 |
| 基因名称 | CENPN |
| Gene ID (NCBI) | 55839 |
| RRID | AB_2878310 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96H22 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CENPN, also named as C16orf60 and ICEN32, is a component of the CENPA-NAC complex. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPN has 4 isoforms with MW 24 kDa and 35-41 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPN antibody 16751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cancer Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement.
| ||
Cancer Rep (Hoboken) High expression of centromere protein N as novel biomarkers for gastric adenocarcinoma
| ||
Horm Metab Res Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway |