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验证数据展示

  • WB analysis of mouse brain using 20611-1-AP

    WB analysis of mouse brain using 20611-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CENPO Polyclonal antibody

CENPO Polyclonal Antibody for WB, ELISA
Cat No. 20611-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CENP O, CENPO, centromere protein O, ICEN36, MCM21R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, HL-60 cells, mouse kidney tissue, mouse ovary tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20611-1-AP targets CENPO in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CENPO fusion protein Ag14619 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centromere protein O
Synonyms CENP O, CENPO, centromere protein O, ICEN36, MCM21R
Calculated Molecular Weight 300 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC002870
Gene Symbol CENPO
Gene ID (NCBI) 79172
RRIDAB_10696179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU64
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for CENPO antibody 20611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols