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验证数据展示

  • WB analysis of mouse lung using 24067-1-AP

    WB analysis of mouse lung using 24067-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24067-1-AP

    WB analysis using 24067-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24067-1-AP

    IF Staining of HeLa using 24067-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • IHC staining of human pancreas using 24067-1-AP

    IHC staining of human pancreas using 24067-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 24067-1-AP

    IHC staining of human pancreas using 24067-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24067-1-AP

    IF Staining of HeLa using 24067-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CEP120 Polyclonal antibody

CEP120 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24067-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ36090, FLJ38327, Coiled-coil domain-containing protein 100, Centrosomal protein of 120 kDa, centrosomal protein 120kDa

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse lung tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24067-1-AP targets CEP120 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CEP120 fusion protein Ag21165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 120kDa
Synonyms FLJ36090, FLJ38327, Coiled-coil domain-containing protein 100, Centrosomal protein of 120 kDa, centrosomal protein 120kDa
Calculated Molecular Weight 986 aa, 113 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC040527
Gene Symbol CEP120
Gene ID (NCBI) 153241
RRIDAB_3085721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N960
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP120 encodes a protein that functions in the microtubule-dependent coupling of the nucleus and the centrosome. It is involved in regulating centrosome-mediated interkinetic nuclear migration of neural progenitors (PMID: 27185865, 23857771). CEP120, SPICE1, and CPAP are required for centriole elongation and for the recruitment of distal microtubule-capping proteins and CEP135 to procentrioles (PMID: 23810536). Cep120 and Taccs are required for the neural progenitor pool during mouse neocortical development (PMID: 17920017).

实验方案

Product Specific Protocols
WB protocol for CEP120 antibody 24067-1-APDownload protocol
IHC protocol for CEP120 antibody 24067-1-APDownload protocol
IF protocol for CEP120 antibody 24067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols