验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, U2OS cells, U-87 MG cells |
| Positive IF/ICC detected in | MDCK cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 8 publications below |
产品信息
24428-1-AP targets CEP135 in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, Canine |
| 文献引用反应性 | human, mouse |
| 免疫原 | CEP135 fusion protein Ag19122 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 135kDa |
| 别名 | centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635 |
| 计算分子量 | 1140 aa, 134 kDa |
| 观测分子量 | 135 kDa |
| GenBank蛋白编号 | BC136535 |
| 基因名称 | CEP135 |
| Gene ID (NCBI) | 9662 |
| RRID | AB_2879543 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q66GS9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP135 antibody 24428-1-AP | Download protocol |
| IF protocol for CEP135 antibody 24428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
Elife WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. | ||
Cells Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells. | ||
J Cell Sci Separation-of-function MCPH-associated mutations in CPAP affect centriole number and length |
