验证数据展示
经过测试的应用
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | hTERT-RPE1 cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18832-1-AP targets CEP192 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IP, IF, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centrosomal protein 192kDa |
别名 | centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein |
计算分子量 | 213 kDa |
观测分子量 | 250-300 kDa |
GenBank蛋白编号 | BC144481 |
基因名称 | CEP192 |
Gene ID (NCBI) | 55125 |
RRID | AB_2878560 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TEP8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.
实验方案
Product Specific Protocols | |
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IHC protocol for CEP192 antibody 18832-1-AP | Download protocol |
IF protocol for CEP192 antibody 18832-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||