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CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for IP, IF, IHC, ELISA
Cat No. 18832-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IP, IF, IHC, ELISA and More (1)

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CEP192 Ready-To-Use IHC Kit. CEP192 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18832-1-AP targets CEP192 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IP, IF, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 192kDa
别名 centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein
计算分子量 213 kDa
观测分子量 250-300 kDa
GenBank蛋白编号BC144481
基因名称 CEP192
Gene ID (NCBI) 55125
RRIDAB_2878560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

实验方案

Product Specific Protocols
IHC protocol for CEP192 antibody 18832-1-APDownload protocol
IF protocol for CEP192 antibody 18832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

Mechanism of spindle pole organization and instability in human oocytes.

Authors - Chun So
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
mouseIF

EMBO Rep

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Authors - Ting Song
humanWB,IF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
mouseIF

PLoS Genet

Aurora kinase A is essential for meiosis in mouse oocytes.

Authors - Cecilia S Blengini
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
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