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验证数据展示

  • WB analysis using 28700-1-AP

    WB analysis using 28700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28700-1-AP (CEP192 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28700-1-AP

    IF Staining of HeLa using 28700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28700-1-AP (CEP192 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28700-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CEP192 Ready-To-Use IHC Kit. CEP192 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28700-1-AP targets CEP192 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 CEP192 fusion protein Ag29930 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 192kDa
别名 centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein
计算分子量 297 kDa
观测分子量280-300 kDa
GenBank蛋白编号NM_032142
基因名称 CEP192
Gene ID (NCBI) 55125
RRIDAB_2918191
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP192, also named PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

实验方案

Product Specific Protocols
WB protocol for CEP192 antibody 28700-1-APDownload protocol
IF protocol for CEP192 antibody 28700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols