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CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28700-1-AP

Print datasheet

Synonyms

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

验证数据展示

  • WB analysis using 28700-1-AP

    WB analysis using 28700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28700-1-AP (CEP192 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28700-1-AP

    IF Staining of HeLa using 28700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28700-1-AP (CEP192 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28700-1-AP targets CEP192 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen CEP192 fusion protein Ag29930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 192kDa
Synonyms centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein
Calculated Molecular Weight 297 kDa
Observed Molecular Weight280-300 kDa
GenBank Accession NumberNM_032142
Gene Symbol CEP192
Gene ID (NCBI) 55125
RRIDAB_2918191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP192, also named PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

实验方案

Product Specific Protocols
WB protocol for CEP192 antibody 28700-1-APDownload protocol
IF protocol for CEP192 antibody 28700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols