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CEP350-Specific Polyclonal antibody

CEP350-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20022-1-AP

Print datasheet

Synonyms

Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa

验证数据展示

  • WB analysis using 20022-1-AP

    WB analysis using 20022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20022-1-AP (CEP350-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 20022-1-AP

    IHC staining of mouse liver using 20022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20022-1-AP (CEP350-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 20022-1-AP

    IF Staining of A431 using 20022-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CEP350-Specific antibody (20022-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20022-1-AP targets CEP350-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 350kDa
Synonyms Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa
Calculated Molecular Weight 351 kDa
Observed Molecular Weight245 kDa
GenBank Accession NumberNM_014810
Gene Symbol CEP350
Gene ID (NCBI) 9857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VT06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP350, or Centrosomal Protein 350 kDa, also named CAP350, FLJ38282, FLJ44058, GM133, and KIAA0480, is a protein that plays a crucial role in the formation and maintenance of the centrosome, which is essential for cell division. The molecular weight of CEP350 varies depending on the isoforms and post-translational modifications. the molecular weight of CEP350 can range from 200-245 kDa, 143 kDa, to 65 kDa. These variations in molecular weight are likely due to different isoforms of the protein that arise from alternative splicing or other post-translational modifications.

实验方案

Product Specific Protocols
WB protocol for CEP350-Specific antibody 20022-1-APDownload protocol
IHC protocol for CEP350-Specific antibody 20022-1-APDownload protocol
IF protocol for CEP350-Specific antibody 20022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols