验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20022-1-AP targets CEP350-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centrosomal protein 350kDa |
别名 | Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa |
计算分子量 | 351 kDa |
观测分子量 | 245 kDa |
GenBank蛋白编号 | NM_014810 |
基因名称 | CEP350 |
Gene ID (NCBI) | 9857 |
RRID | AB_11232427 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q5VT06 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP350, or Centrosomal Protein 350 kDa, also named CAP350, FLJ38282, FLJ44058, GM133, and KIAA0480, is a protein that plays a crucial role in the formation and maintenance of the centrosome, which is essential for cell division. The molecular weight of CEP350 varies depending on the isoforms and post-translational modifications. the molecular weight of CEP350 can range from 200-245 kDa, 143 kDa, to 65 kDa. These variations in molecular weight are likely due to different isoforms of the protein that arise from alternative splicing or other post-translational modifications.
实验方案
Product Specific Protocols | |
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WB protocol for CEP350-Specific antibody 20022-1-AP | Download protocol |
IHC protocol for CEP350-Specific antibody 20022-1-AP | Download protocol |
IF protocol for CEP350-Specific antibody 20022-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |