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- KD/KO Validated
CEP55 Polyclonal antibody
CEP55 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, rat testis tissue, human testis tissue, HeLa cells, mouse testis tissue, Jurkat cells |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | CEP55 fusion protein Ag20940 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | centrosomal protein 55kDa |
Synonyms | C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, Up regulated in colon cancer 6, URCC6 |
Calculated Molecular Weight | 54 kDa |
Observed Molecular Weight | 47-55 kDa |
GenBank Accession Number | BC008947 |
Gene Symbol | CEP55 |
Gene ID (NCBI) | 55165 |
RRID | AB_2802169 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | Q53EZ4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CEP55 antibody 23891-1-AP | Download protocol |
IHC protocol for CEP55 antibody 23891-1-AP | Download protocol |
IF protocol for CEP55 antibody 23891-1-AP | Download protocol |
IP protocol for CEP55 antibody 23891-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
| ||
Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. | ||
Front Oncol USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.
| ||
Cells CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.
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iScience Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission. |