验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, human testis tissue, mouse testis tissue, Jurkat cells |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | CEP55 fusion protein Ag20940 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centrosomal protein 55kDa |
别名 | |
计算分子量 | 54 kDa |
观测分子量 | 47-55 kDa |
GenBank蛋白编号 | BC008947 |
基因名称 | CEP55 |
Gene ID (NCBI) | 55165 |
RRID | AB_2802169 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen Affinity purified |
UNIPROT ID | Q53EZ4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for CEP55 antibody 23891-1-AP | Download protocol |
IHC protocol for CEP55 antibody 23891-1-AP | Download protocol |
IF protocol for CEP55 antibody 23891-1-AP | Download protocol |
IP protocol for CEP55 antibody 23891-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
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Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. | ||
Front Oncol USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.
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Cells CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.
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iScience Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission. |