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  • KD/KO Validated

CEP55 Polyclonal antibody

CEP55 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23891-1-AP

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Synonyms

C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, Up regulated in colon cancer 6, URCC6



经过测试的应用

Positive WB detected inHEK-293 cells, rat testis tissue, human testis tissue, HeLa cells, mouse testis tissue, Jurkat cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CEP55 fusion protein Ag20940 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 55kDa
Synonyms C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, Up regulated in colon cancer 6, URCC6
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 47-55 kDa
GenBank Accession NumberBC008947
Gene Symbol CEP55
Gene ID (NCBI) 55165
RRIDAB_2802169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ53EZ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

实验方案

Product Specific Protocols
WB protocol for CEP55 antibody 23891-1-APDownload protocol
IHC protocol for CEP55 antibody 23891-1-APDownload protocol
IF protocol for CEP55 antibody 23891-1-APDownload protocol
IP protocol for CEP55 antibody 23891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
humanWB,IP,IF

Cell Death Dis

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Authors - Kun Gao
  • KD Validated
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
humanWB

Front Oncol

USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.

Authors - Catherine M Nickson
  • KD Validated
humanWB,IHC

Cells

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Authors - Minjing Li
  • KD Validated
humanIF

iScience

Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission.

Authors - Kangji Wang