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CEP55 Polyclonal antibody

CEP55 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 23891-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human testis tissue, mouse testis tissue, Jurkat cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CEP55 Ready-To-Use IHC Kit. CEP55 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 CEP55 fusion protein Ag20940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 55kDa
别名
计算分子量 54 kDa
观测分子量 47-55 kDa
GenBank蛋白编号BC008947
基因名称 CEP55
Gene ID (NCBI) 55165
RRIDAB_2802169
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ53EZ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

实验方案

Product Specific Protocols
WB protocol for CEP55 antibody 23891-1-APDownload protocol
IHC protocol for CEP55 antibody 23891-1-APDownload protocol
IF protocol for CEP55 antibody 23891-1-APDownload protocol
IP protocol for CEP55 antibody 23891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
humanWB,IP,IF

Cell Death Dis

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Authors - Kun Gao
  • KD Validated
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
humanWB

Front Oncol

USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.

Authors - Catherine M Nickson
  • KD Validated
humanWB,IHC

Cells

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Authors - Minjing Li
  • KD Validated
humanIF

iScience

Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission.

Authors - Kangji Wang
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