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  • KD/KO Validated

CEP72 Polyclonal antibody

CEP72 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19928-1-AP

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Synonyms

KIAA1519, Centrosomal protein of 72 kDa, centrosomal protein 72kDa



经过测试的应用

Positive WB detected inmouse testis tissue, HEK-293 cells, rat testis tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19928-1-AP targets CEP72 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CEP72 fusion protein Ag13905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 72kDa
Synonyms KIAA1519, Centrosomal protein of 72 kDa, centrosomal protein 72kDa
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC001750
Gene Symbol CEP72
Gene ID (NCBI) 55722
RRIDAB_10638607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CEP72 antibody 19928-1-APDownload protocol
IF protocol for CEP72 antibody 19928-1-APDownload protocol
IP protocol for CEP72 antibody 19928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IP, IF

Elife

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Authors - Andrew Kodani
  • KD Validated
humanIF

EMBO Rep

Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.

Authors - Andrea Martello
humanIF

J Cell Sci

Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Authors - Deniz Conkar
humanWB

Sci Rep

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Authors - Deniz Conkar
  • KD Validated
humanIF

J Biol Chem

Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131).

Authors - Ryan A Denu
humanWB,IF

BMC Biol

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72

Authors - Zhenguang Ying