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CEP72 Polyclonal antibody
CEP72 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, HEK-293 cells, rat testis tissue |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19928-1-AP targets CEP72 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | CEP72 fusion protein Ag13905 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | centrosomal protein 72kDa |
Synonyms | KIAA1519, Centrosomal protein of 72 kDa, centrosomal protein 72kDa |
Calculated Molecular Weight | 646 aa, 72 kDa |
Observed Molecular Weight | 72 kDa |
GenBank Accession Number | BC001750 |
Gene Symbol | CEP72 |
Gene ID (NCBI) | 55722 |
RRID | AB_10638607 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9P209 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CEP72 antibody 19928-1-AP | Download protocol |
IF protocol for CEP72 antibody 19928-1-AP | Download protocol |
IP protocol for CEP72 antibody 19928-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
| ||
EMBO Rep Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy. | ||
J Cell Sci Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. | ||
Sci Rep Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.
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J Biol Chem Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131). | ||
BMC Biol CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72 |