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验证数据展示

  • WB analysis of A549 using 30165-1-AP

    WB analysis of A549 using 30165-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 30165-1-AP (CCDC123 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 30165-1-AP

    IF Staining of hTERT-RPE1 using 30165-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CCDC123 antibody (30165-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CEP89, CCDC123 Polyclonal antibody

CEP89, CCDC123 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30165-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CEP89, CCDC123, Coiled-coil domain-containing protein 123, Cep123, Centrosomal protein of 89 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30165-1-AP targets CEP89, CCDC123 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CEP89, CCDC123 fusion protein Ag32789 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 123
别名 CEP89, CCDC123, Coiled-coil domain-containing protein 123, Cep123, Centrosomal protein of 89 kDa
计算分子量 783 aa, 90 kDa
观测分子量 85-100 kDa
GenBank蛋白编号BC136328
基因名称 CEP89
Gene ID (NCBI) 84902
RRIDAB_3669704
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96ST8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.

实验方案

Product Specific Protocols
WB protocol for CEP89, CCDC123 antibody 30165-1-APDownload protocol
IF protocol for CEP89, CCDC123 antibody 30165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols