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CER1 Polyclonal antibody

CER1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 18879-1-AP

Print datasheet

Synonyms

CER1, Cerberus, Cerberus-related protein, DAND4

验证数据展示

  • WB analysis using 18879-1-AP

    WB analysis using 18879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18879-1-AP (CER1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 18879-1-AP

    IF Staining of mouse kidney using 18879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CER1 antibody (18879-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inU-251 cells, mouse kidney tissue, rat kidney tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18879-1-AP targets CER1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CER1 fusion protein Ag12926 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
Synonyms CER1, Cerberus, Cerberus-related protein, DAND4
Calculated Molecular Weight 267 aa, 30 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC069405
Gene Symbol CER1
Gene ID (NCBI) 9350
RRIDAB_3085568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. CER1 can exist as homo- or heterodimers (PMID: 23717584, 9501024).

实验方案

Product Specific Protocols
WB protocol for CER1 antibody 18879-1-APDownload protocol
IF protocol for CER1 antibody 18879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols