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CER1 Polyclonal antibody

CER1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 18879-1-AP

Print datasheet

Synonyms

CER1, Cerberus, Cerberus-related protein, DAND4

验证数据展示

  • WB analysis using 18879-1-AP

    WB analysis using 18879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18879-1-AP (CER1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 18879-1-AP

    IF Staining of mouse kidney using 18879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CER1 antibody (18879-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inU-251 cells, mouse kidney tissue, rat kidney tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18879-1-AP targets CER1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CER1 fusion protein Ag12926 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
Synonyms CER1, Cerberus, Cerberus-related protein, DAND4
Calculated Molecular Weight 267 aa, 30 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC069405
Gene Symbol CER1
Gene ID (NCBI) 9350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. CER1 can exist as homo- or heterodimers (PMID: 23717584, 9501024).

实验方案

Product Specific Protocols
WB protocol for CER1 antibody 18879-1-APDownload protocol
IF protocol for CER1 antibody 18879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols