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CERK Polyclonal antibody, PBS Only

CERK Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25731-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

产品信息

25731-1-PBS targets CERK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CERK fusion protein Ag22774 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ceramide kinase
Synonyms
Calculated Molecular Weight 537 aa, 60 kDa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC126940
Gene Symbol CERK
Gene ID (NCBI) 64781
RRIDAB_2880214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCT0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.