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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

CERK Polyclonal antibody, PBS Only

CERK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25731-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

25731-1-PBS targets CERK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CERK fusion protein Ag22774 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ceramide kinase
别名
计算分子量 537 aa, 60 kDa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC126940
基因名称 CERK
Gene ID (NCBI) 64781
RRIDAB_2880214
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCT0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.