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CES1 Polyclonal antibody, PBS Only

CES1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29041-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 29041-1-AP (same clone as 29041-1-PBS)

    WB analysis using 29041-1-AP (same clone as 29041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 29041-1-AP (same clone as 29041-1-PBS)

    IF Staining of C2C12 using 29041-1-AP (same clone as 29041-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 29041-1-AP (same clone as 29041-1-PBS)

    IHC staining of mouse liver using 29041-1-AP (same clone as 29041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29041-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CES1 antibody included
  • IF Staining of HepG2 using 29041-1-AP (same clone as 29041-1-PBS)

    IF Staining of HepG2 using 29041-1-AP (same clone as 29041-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.

产品信息

29041-1-PBS targets CES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CES1 fusion protein Ag30534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Synonyms
Calculated Molecular Weight 566 aa, 62 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23141
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human carboxylesterase 1 (hCE1 or CES1) is a serine esterase that hydrolyzes a variety of endogenous and exogenous substrates. CES1 contributes to approximately 90% of hepatic hydrolytic activity in human livers (PMID: 27895113). Variation in CES1 has the ability to affect the metabolism of methylphenidate (PMID: 28087982). CES1 has 567 amino acids and a theoretical molecular mass of 62 kDa.