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CES3 Polyclonal antibody

CES3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14587-1-AP

Print datasheet

Synonyms

UNQ869/PRO1887, ES31, EC:3.1.1.1, Carboxylesterase 3

验证数据展示

  • WB analysis using 14587-1-AP

    WB analysis using 14587-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14587-1-AP (CES3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14587-1-AP

    IF Staining of HeLa using 14587-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14587-1-AP (CES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 14587-1-AP

    IHC staining of human stomach cancer using 14587-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14587-1-AP (CES3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14587-1-AP targets CES3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CES3 fusion protein Ag6113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxylesterase 3
Synonyms UNQ869/PRO1887, ES31, EC:3.1.1.1, Carboxylesterase 3
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 60-62 kDa
GenBank Accession NumberBC053670
Gene Symbol CES3
Gene ID (NCBI) 23491
RRIDAB_2275992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is expressed in liver, colon and small intestine. This antibody recognizes both isoforms with MW 60-62 kDa and 24 kDa.

实验方案

Product Specific Protocols
WB protocol for CES3 antibody 14587-1-APDownload protocol
IHC protocol for CES3 antibody 14587-1-APDownload protocol
IF protocol for CES3 antibody 14587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Proteome Res

Profiling impaired hepatic endoplasmic reticulum glycosylation as a consequence of ethanol ingestion.

Authors - Galligan James J JJ
View Article
humanWB

Environ Toxicol Pharmacol

Formaldehyde alters triglyceride synthesis and very low-density lipoprotein secretion in a time-dependent manner.

Authors - Jianying Bai
View Article