Centrin 1 Polyclonal antibody

Centrin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12794-1-AP

Print datasheet

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2



经过测试的应用

Positive WB detected inHL-60 cells, human heart tissue
Positive IF/ICC detected inhTERT-RPE1 cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12794-1-AP targets Centrin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Immunogen Centrin 1 fusion protein Ag3529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrin, EF-hand protein, 1
Synonyms CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC029515
Gene Symbol Centrin 1
Gene ID (NCBI) 1068
RRIDAB_2077371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

实验方案

Product Specific Protocols
WB protocol for Centrin 1 antibody 12794-1-APDownload protocol
IF protocol for Centrin 1 antibody 12794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB,IF

Nat Cell Biol

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Authors - Huijie Zhao
human

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
humanIF

Cell Res

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Authors - Cheng Zhang
mouseIF

Nat Commun

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Authors - Munemasa Mori
zebrafishIF

Nucleic Acids Res

Resting cells rely on the DNA helicase component MCM2 to build cilia.

Authors - Teresa Casar Tena
humanIF

Nat Commun

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Authors - Xiaowei Xu