验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, human heart tissue |
Positive IF/ICC detected in | hTERT-RPE1 cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12794-1-AP targets Centrin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse, zebrafish |
免疫原 | Centrin 1 fusion protein Ag3529 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | centrin, EF-hand protein, 1 |
别名 | CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2 |
计算分子量 | 172 aa, 20 kDa |
观测分子量 | 20 kDa |
GenBank蛋白编号 | BC029515 |
基因名称 | Centrin 1 |
Gene ID (NCBI) | 1068 |
RRID | AB_2077371 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q12798 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Centrin 1 antibody 12794-1-AP | Download protocol |
IF protocol for Centrin 1 antibody 12794-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. | ||
Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5. | ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
Nat Commun DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6. |