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CETP Polyclonal antibody

CETP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30136-1-AP

Print datasheet

Synonyms

CETP, HDLCQ10, Lipid transfer protein I

验证数据展示

  • WB analysis using 30136-1-AP

    WB analysis using 30136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30136-1-AP (CETP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, HT-29 cells, HepG2 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30136-1-AP targets CETP in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen CETP fusion protein Ag32722 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cholesteryl ester transfer protein, plasma
Synonyms CETP, HDLCQ10, Lipid transfer protein I
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight55 kDa,70 kDa
GenBank Accession NumberBC025739
Gene Symbol CETP
Gene ID (NCBI) 1071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cholesteryl ester transfer protein (CETP) is a plasma glycoprotein which transfers cholesteryl esters, triglycerides, and phospholipids between lipoproteins. In plasma, CETP is mainly associated with HDL particles and catalyzes the transfer of cholesteryl esters from HDL to apolipoprotein (apo) B-containing plasma lipoproteins. Low plasma concentrations of high-density lipoprotein-cholesterol (HDL-C) have long been recognized as a major, independent cardiovascular disease (CVD) risk factor. It demonstrated that altered level of plasma CETP was associated with the increased risk of CVD (PMID: 17991755, PMID: 23477743). CETP can be detected a 70 kDa isoform by glycosylation (PMID: 3281933,PMID: 22056562). It also can be detected 100 kDa dimer (PMID: 7744792).

实验方案

Product Specific Protocols
WB protocol for CETP antibody 30136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols