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验证数据展示

  • IP experiment of mouse testis using 31344-1-AP

    IP experiment of mouse testis using 31344-1-AP

    IP result of anti-CFAP70 (IP:31344-1-AP, 4ug; Detection:31344-1-AP 1:300) with mouse testis tissue lysate 1120 ug.

  • IHC staining of rat testis using 31344-1-AP

    IHC staining of rat testis using 31344-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 31344-1-AP

    IHC staining of mouse testis using 31344-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 31344-1-AP

    IF Staining of mouse testis using 31344-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CFAP70 antibody (31344-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CFAP70 Polyclonal antibody

CFAP70 Polyclonal Antibody for IHC, IF-P, IP, ELISA
Cat No. 31344-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, IP, ELISA

别名

TTC18, TPR repeat protein 18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inmouse testis tissue
Positive IHC detected inrat testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31344-1-AP targets CFAP70 in IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CFAP70 fusion protein Ag35209 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 18
别名 TTC18, TPR repeat protein 18
计算分子量126 kDa
观测分子量 126 kDa
GenBank蛋白编号BC032856
基因名称 TTC18
Gene ID (NCBI) 118491
RRIDAB_3669954
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T0N1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cilia- and flagella-associated protein 70 (CFAP70) harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).

实验方案

Product Specific Protocols
IHC protocol for CFAP70 antibody 31344-1-APDownload protocol
IF protocol for CFAP70 antibody 31344-1-APDownload protocol
IP protocol for CFAP70 antibody 31344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols