Complement factor B Polyclonal antibody
Complement factor B Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, human blood, rat plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10170-1-AP targets Complement factor B in WB, IHC, ELISA, IF applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Complement factor B fusion protein Ag0226 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | complement factor B |
| Synonyms | CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 93-100 kDa, 60-69 kDa |
| GenBank Accession Number | BC007990 |
| Gene Symbol | Complement factor B |
| Gene ID (NCBI) | 629 |
| RRID | AB_2082384 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00751 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Complement factor B antibody 10170-1-AP | Download protocol |
| IHC protocol for Complement factor B antibody 10170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The classical pathway triggers pathogenic complement activation in membranous nephropathy | ||
J Cell Physiol IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium. | ||
Cell Oncol (Dordr) Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer. | ||
Br J Cancer Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins. | ||
Nephrol Dial Transplant Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation. | ||
Chem Res Toxicol 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells. |
