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Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11848-1-AP

Print datasheet

Synonyms

CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2

验证数据展示

  • WB analysis of human liver using 11848-1-AP

    WB analysis of human liver using 11848-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 11848-1-AP

    IHC staining of human skeletal muscle using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CFL2 antibody included
  • IF Staining of L02 using 11848-1-AP

    IF Staining of L02 using 11848-1-AP

    Immunofluorescent analysis of L02 cells using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human normal colon using 11848-1-AP

    IHC staining of human normal colon using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CFL2 antibody included
  • IHC staining of human normal colon using 11848-1-AP

    IHC staining of human normal colon using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CFL2 antibody included

经过测试的应用

Positive WB detected inhuman liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman normal colon, human gliomas tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy CFL2 Ready-To-Use IHC Kit. CFL2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11848-1-AP targets Cofilin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Cofilin 2 fusion protein Ag2408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cofilin 2 (muscle)
Synonyms CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2
Calculated Molecular Weight 166 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC022876
Gene Symbol Cofilin 2
Gene ID (NCBI) 1073
RRIDAB_2080914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y281
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IHC protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IF protocol for Cofilin 2 antibody 11848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.

Authors - Lara Ottaviani
View Article
humanWB,IHC,IF

Noncoding RNA Res

ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma

Authors - Haoyuan Xu
View Article