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验证数据展示

  • WB analysis of human liver using 11848-1-AP

    WB analysis of human liver using 11848-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 11848-1-AP

    IHC staining of human skeletal muscle using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CFL2 antibody included
  • IHC staining of human colon using 11848-1-AP

    IHC staining of human colon using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CFL2 antibody included
  • IHC staining of human colon using 11848-1-AP

    IHC staining of human colon using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CFL2 antibody included
  • IF Staining of L02 using 11848-1-AP

    IF Staining of L02 using 11848-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Cofilin 2 antibody (11848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11848-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman normal colon, human gliomas tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy CFL2 Ready-To-Use IHC Kit. CFL2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11848-1-AP targets Cofilin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Cofilin 2 fusion protein Ag2408 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cofilin 2 (muscle)
别名 CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2
计算分子量 166 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC022876
基因名称 Cofilin 2
Gene ID (NCBI) 1073
RRIDAB_2080914
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y281
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IHC protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IF protocol for Cofilin 2 antibody 11848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.

Authors - Lara Ottaviani
View Article
humanWB,IHC,IF

Noncoding RNA Res

ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma

Authors - Haoyuan Xu
View Article