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CoraLite® Plus 488-conjugated CFP Monoclonal antibody

CFP Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5A9

Cat no : CL488-66788

Print datasheet

Synonyms

BFD, CFP, Complement factor P, complement factor properdin, PFC, PFD, PROPERDIN

验证数据展示

  • WB analysis using CL488-66788

    WB analysis using CL488-66788

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66788 (CFP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66788-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66788 targets CFP in WB applications and shows reactivity with Human, pig, rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, pig, rat
Immunogen CFP fusion protein Ag9798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement factor properdin
Synonyms BFD, CFP, Complement factor P, complement factor properdin, PFC, PFD, PROPERDIN
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 50 kDa, 100 kDa, 150 kDa
GenBank Accession NumberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27918
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105). In plasma, properdin exists as dimers, trimers or tetramers.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CFP antibody CL488-66788Download protocol
Standard Protocols
Click here to view our Standard Protocols