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  • Featured Product
  • KD/KO Validated

CGGBP1 Polyclonal antibody

CGGBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 10716-1-AP

Print datasheet

Synonyms

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

验证数据展示

  • WB analysis using 10716-1-AP

    WB analysis using 10716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10716-1-AP

    IHC staining of human breast cancer using 10716-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10716-1-AP (CGGBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CGGBP1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, mouse thymus tissue, K-562 cells, Raji cells, HL-60 cells, Jurkat cells, rat thymus tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CGGBP1 Ready-To-Use IHC Kit. CGGBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10716-1-AP targets CGGBP1 in WB, IHC, IF, IP, chIP, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IP, chIP, Dot Blot
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CGGBP1 fusion protein Ag1129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CGG triplet repeat binding protein 1
Synonyms 20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19-23 kDa
GenBank Accession NumberBC005222
Gene Symbol CGGBP1
Gene ID (NCBI) 8545
RRIDAB_2079283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

实验方案

Product Specific Protocols
WB protocol for CGGBP1 antibody 10716-1-APDownload protocol
IHC protocol for CGGBP1 antibody 10716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Cell Cycle

CGGBP1 phosphorylation constitutes a telomere-protection signal.

Authors - Umashankar Singh
  • KD Validated
View Article
humanchIP

Cell Cycle

Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs.

Authors - Prasoon Agarwal
View Article
humanWB,IP

Epigenetics Chromatin

CGGBP1 regulates CTCF occupancy at repeats.

Authors - Divyesh Patel
View Article
humanIF

Exp Cell Res

CGGBP1 is a nuclear and midbody protein regulating abscission.

Authors - Singh Umashankar U
View Article
humanWB,Dot Blot

BMC Cancer

An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1.

Authors - Manthan Patel
View Article
humanWB

BMC Genet

CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.

Authors - Manthan Patel
View Article