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CGNL1 Polyclonal antibody

CGNL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18031-1-AP

Print datasheet

Synonyms

CGNL1, cingulin like 1, Cingulin like protein 1, JACOP, KIAA1749, Paracingulin

验证数据展示

  • WB analysis of NIH/3T3 using 18031-1-AP

    WB analysis of NIH/3T3 using 18031-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18031-1-AP (CGNL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18031-1-AP

    IHC staining of human liver cancer using 18031-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18031-1-AP (CGNL1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 18031-1-AP

    IHC staining of human liver cancer using 18031-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18031-1-AP (CGNL1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18031-1-AP targets CGNL1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CGNL1 fusion protein Ag12598 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cingulin-like 1
Synonyms CGNL1, cingulin like 1, Cingulin like protein 1, JACOP, KIAA1749, Paracingulin
Calculated Molecular Weight 1302 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC093827
Gene Symbol CGNL1
Gene ID (NCBI) 84952
RRIDAB_10644446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VF96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CGNL1 antibody 18031-1-APDownload protocol
IHC protocol for CGNL1 antibody 18031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF,IHC

Cell Rep

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease

Authors - Min Yu
View Article
humanWB

Sci Rep

Single-cell and bulk RNA sequencing reveals Anoikis related genes to guide prognosis and immunotherapy in osteosarcoma

Authors - Cheng Zhong
View Article