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CGRRF1 Polyclonal antibody

CGRRF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16101-1-AP

Print datasheet

Synonyms

CGR19, CGRRF1, RING finger protein 197, RNF197

验证数据展示

  • WB analysis of rat kidney using 16101-1-AP

    WB analysis of rat kidney using 16101-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 16101-1-AP (CGRRF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat kidney tissue, L02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16101-1-AP targets CGRRF1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen CGRRF1 fusion protein Ag8998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell growth regulator with ring finger domain 1
Synonyms CGR19, CGRRF1, RING finger protein 197, RNF197
Calculated Molecular Weight 332 aa, 38 kDa
Observed Molecular Weight 42-48 kDa
GenBank Accession NumberBC015063
Gene Symbol CGRRF1
Gene ID (NCBI) 10668
RRIDAB_2878219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99675
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CGRRF1 is a growth suppressor and consists of a transmembrane domain and a RING-finger domain. It functions as a RING domain E3 ubiquitin ligase involved in endoplasmic reticulum-associated degradation. The expression of CGRRF1 is decreased in cancer tissues.(PMID: 31801577).

实验方案

Product Specific Protocols
WB protocol for CGRRF1 antibody 16101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols