• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IP experiment of mouse liver using 16734-1-AP

    IP experiment of mouse liver using 16734-1-AP

    IP result of anti-CH25H (IP:16734-1-AP, 4ug; Detection:16734-1-AP 1:300) with mouse liver tissue lysate 5200ug.

  • IHC staining of mouse liver using 16734-1-AP

    IHC staining of mouse liver using 16734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16734-1-AP (CH25H Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 16734-1-AP

    IHC staining of mouse liver using 16734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16734-1-AP (CH25H Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CH25H Polyclonal antibody

CH25H Polyclonal Antibody for IP, IHC, ELISA
Cat No. 16734-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IP, IHC, ELISA

别名

C25H, CH25H, cholesterol 25 hydroxylase, Cholesterol 25 monooxygenase, h25OH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16734-1-AP targets CH25H in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CH25H fusion protein Ag9706 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cholesterol 25-hydroxylase
别名 C25H, CH25H, cholesterol 25 hydroxylase, Cholesterol 25 monooxygenase, h25OH
计算分子量 272 aa, 32 kDa
GenBank蛋白编号BC017843
基因名称 CH25H
Gene ID (NCBI) 9023
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95992
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.