验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CHAF1B Ready-To-Use IHC Kit. CHAF1B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| ChIP | See 1 publications below |
产品信息
27633-1-AP targets CHAF1B in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CHAF1B fusion protein Ag26504 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromatin assembly factor 1, subunit B (p60) |
| 别名 | MPP 7, p60 CAF1, CAF1, CAF IP60, CAF I p60 |
| 计算分子量 | 559 aa, 61 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC021218 |
| 基因名称 | CHAF1B |
| Gene ID (NCBI) | 8208 |
| RRID | AB_2880933 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13112 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHAF1B antibody 27633-1-AP | Download protocol |
| IHC protocol for CHAF1B antibody 27633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.
| ||
Cancer Res Commun Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells |
