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CHCHD1 Polyclonal antibody

CHCHD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 11728-1-AP

Print datasheet

Synonyms

Nuclear protein C2360, MRP-S37, MRPS37, Coiled-coil-helix-coiled-coil-helix domain-containing protein 1, C10orf34

验证数据展示

  • WB analysis of A431 using 11728-1-AP

    WB analysis of A431 using 11728-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11728-1-AP (CHCHD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 11728-1-AP

    FC experiment of A431 using 11728-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CHCHD1 Polyclonal antibody (11728-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using 11728-1-AP

    IF Staining of A431 using 11728-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 11728-1-AP (CHCHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 11728-1-AP

    IHC staining of human stomach cancer using 11728-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11728-1-AP (CHCHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11728-1-AP targets CHCHD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CHCHD1 fusion protein Ag2325 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 1
Synonyms Nuclear protein C2360, MRP-S37, MRPS37, Coiled-coil-helix-coiled-coil-helix domain-containing protein 1, C10orf34
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC020852
Gene Symbol CHCHD1
Gene ID (NCBI) 118487
RRIDAB_2079769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHCHD1 antibody 11728-1-APDownload protocol
IHC protocol for CHCHD1 antibody 11728-1-APDownload protocol
IF protocol for CHCHD1 antibody 11728-1-APDownload protocol
FC protocol for CHCHD1 antibody 11728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

iScience

Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

Authors - Taru Hilander
View Article