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  • Featured Product
  • KD/KO Validated

CHCHD2 Recombinant antibody

CHCHD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A12

Cat no : 82702-1-RR

Print datasheet

Synonyms

HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10

验证数据展示

  • WB analysis using 82702-1-RR

    WB analysis using 82702-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82702-1-RR (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82702-1-RR

    WB analysis of HEK-293 using 82702-1-RR

    WB result of CHCHD2 antibody (82702-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

  • IF Staining of HepG2 using 82702-1-RR

    IF Staining of HepG2 using 82702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (82702-1-RR, Clone: 4A12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82702-1-RR

    Affinity and Kinetic Characterization of 82702-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82702-1-RR against Human CHCHD2 were performed. The affinity constant is 2.12 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82702-1-RR targets CHCHD2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen CHCHD2 fusion protein Ag13752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Synonyms HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10
Calculated Molecular Weight 151 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003079
Gene Symbol CHCHD2
Gene ID (NCBI) 51142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6H1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

实验方案

Product Specific Protocols
WB protocol for CHCHD2 antibody 82702-1-RRDownload protocol
IF protocol for CHCHD2 antibody 82702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols