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验证数据展示

  • WB analysis using 82702-1-RR

    WB analysis using 82702-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82702-1-RR (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82702-1-RR

    WB analysis of HEK-293 using 82702-1-RR

    WB result of CHCHD2 antibody (82702-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

  • IF Staining of HepG2 using 82702-1-RR

    IF Staining of HepG2 using 82702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (82702-1-RR, Clone: 4A12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82702-1-RR

    Affinity and Kinetic Characterization of 82702-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82702-1-RR against Human CHCHD2 were performed. The affinity constant is 2.12 nM.

  • Featured Product
  • KD/KO验证

CHCHD2 Recombinant antibody

CHCHD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82702-1-RR

产品说明书

CloneNo. 4A12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82702-1-RR targets CHCHD2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 CHCHD2 fusion protein Ag13752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 2
别名 HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10
计算分子量 151 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC003079
基因名称 CHCHD2
Gene ID (NCBI) 51142
RRIDAB_3086512
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6H1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

实验方案

Product Specific Protocols
WB protocol for CHCHD2 antibody 82702-1-RRDownload protocol
IF protocol for CHCHD2 antibody 82702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols