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CHCHD4 Monoclonal antibody, PBS Only

CHCHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4C6

Cat no : 66718-1-PBS

Print datasheet

Synonyms

CHCHD4, FLJ31709, MIA40

验证数据展示

  • IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

    IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using CHCHD4 antibody (66718-1-Ig, Clone: 3E4C6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

    WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

产品信息

66718-1-PBS targets CHCHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CHCHD4 fusion protein Ag15339 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 4
Synonyms CHCHD4, FLJ31709, MIA40
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033775
Gene Symbol CHCHD4
Gene ID (NCBI) 131474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N4Q1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).