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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 25711-1-AP

    WB analysis using 25711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25711-1-AP (CHCHD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 25711-1-AP

    IF Staining of MCF-7 using 25711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD5 antibody (25711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CHCHD5 Polyclonal antibody

CHCHD5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25711-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C2orf9, CHCHD5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25711-1-AP targets CHCHD5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CHCHD5 fusion protein Ag22503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 5
Synonyms C2orf9, CHCHD5
Calculated Molecular Weight 110 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC004498
Gene Symbol CHCHD5
Gene ID (NCBI) 84269
RRIDAB_3085818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHCHD5 antibody 25711-1-APDownload protocol
IF protocol for CHCHD5 antibody 25711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols