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CHCHD5 Polyclonal antibody

CHCHD5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 25711-1-AP

Print datasheet

Synonyms

C2orf9, CHCHD5

验证数据展示

  • WB analysis using 25711-1-AP

    WB analysis using 25711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25711-1-AP (CHCHD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 25711-1-AP

    IF Staining of MCF-7 using 25711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD5 antibody (25711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25711-1-AP targets CHCHD5 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CHCHD5 fusion protein Ag22503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 5
Synonyms C2orf9, CHCHD5
Calculated Molecular Weight 110 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC004498
Gene Symbol CHCHD5
Gene ID (NCBI) 84269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHCHD5 antibody 25711-1-APDownload protocol
IF protocol for CHCHD5 antibody 25711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols