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验证数据展示

  • WB analysis using 66584-1-Ig

    WB analysis using 66584-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66584-1-Ig (CHCHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human ovary tumor using 66584-1-Ig

    IHC staining of human ovary tumor using 66584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66584-1-Ig (CHCHD5 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 66584-1-Ig

    IHC staining of human ovary tumor using 66584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66584-1-Ig (CHCHD5 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHCHD5 Monoclonal antibody

CHCHD5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66584-1-Ig

产品说明书

CloneNo. 2G10A7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

C2orf9, CHCHD5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, THP-1 cells, HL-60 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66584-1-Ig targets CHCHD5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 CHCHD5 fusion protein Ag22534 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 5
别名 C2orf9, CHCHD5
计算分子量 110 aa, 12 kDa
观测分子量 14 kDa
GenBank蛋白编号BC004498
基因名称 CHCHD5
Gene ID (NCBI) 84269
RRIDAB_2881944
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BSY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHCHD5 antibody 66584-1-IgDownload protocol
IHC protocol for CHCHD5 antibody 66584-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols