验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20639-1-AP targets CHCHD6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | CHCHD6 fusion protein Ag14700 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | coiled-coil-helix-coiled-coil-helix domain containing 6 |
别名 | MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1 |
计算分子量 | 235 aa, 26 kDa |
观测分子量 | 26-29 kDa |
GenBank蛋白编号 | BC006123 |
基因名称 | CHCHD6 |
Gene ID (NCBI) | 84303 |
RRID | AB_10697667 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BRQ6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
IHC protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
IF protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
IP protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Elife QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. | ||
EMBO Rep Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner. | ||
Elife QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease. | ||
Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
| ||
Sci Rep Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.
| ||
Biochim Biophys Acta Biomembr Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes. |