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CHCHD6 Polyclonal antibody

CHCHD6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20639-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20639-1-AP targets CHCHD6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 CHCHD6 fusion protein Ag14700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 6
别名 MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1
计算分子量 235 aa, 26 kDa
观测分子量 26-29 kDa
GenBank蛋白编号BC006123
基因名称 CHCHD6
Gene ID (NCBI) 84303
RRIDAB_10697667
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHCHD6 antibody 20639-1-APDownload protocol
IHC protocol for CHCHD6 antibody 20639-1-APDownload protocol
IF protocol for CHCHD6 antibody 20639-1-APDownload protocol
IP protocol for CHCHD6 antibody 20639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani
humanWB

EMBO Rep

Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

Authors - Arun Kumar Kondadi
humanWB

Elife

QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.

Authors - Virginia Guarani
mouseWB

Cell Death Differ

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Authors - H Li
  • KD Validated
humanWB

Sci Rep

Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Authors - Chengli Ding
  • KO Validated
humanWB

Biochim Biophys Acta Biomembr

Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.

Authors - Jennifer Urbach
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