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CHD2 Polyclonal antibody

CHD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat No : 12311-1-AP

Print datasheet

Synonyms

ATP dependent helicase CHD2, CHD 2, CHD2

验证数据展示

  • WB analysis of human placenta using 12311-1-AP

    WB analysis of human placenta using 12311-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12311-1-AP (CHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12311-1-AP

    IHC staining of human lymphoma using 12311-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12311-1-AP (CHD2 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of human placenta using 12311-1-AP

    IP experiment of human placenta using 12311-1-AP

    IP result of anti-CHD2 (IP:12311-1-AP, 4ug; Detection:12311-1-AP 1:300) with human placenta tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12311-1-AP targets CHD2 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman, rat
Immunogen CHD2 fusion protein Ag2960 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromodomain helicase DNA binding protein 2
Synonyms ATP dependent helicase CHD2, CHD 2, CHD2
Calculated Molecular Weight 1828 aa, 211 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC020810
Gene Symbol CHD2
Gene ID (NCBI) 1106
RRIDAB_2079925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromodomain-helicase-DNA-binding protein 2, also named as ATP-dependent helicase CHD2, CHD2. It is a sequence-selective DNA-binding protein. Molecular mass of CHD2 is 211kD (201kd for isoform 2, 57kd for isoform 3). CHD2 is confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage.

实验方案

Product Specific Protocols
WB protocol for CHD2 antibody 12311-1-APDownload protocol
IHC protocol for CHD2 antibody 12311-1-APDownload protocol
IP protocol for CHD2 antibody 12311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Neuron

Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.

Authors - Zhixiong Liu
View Article
humanWB

PeerJ

Identification and validation of immune related core transcription factors GTF2I in NAFLD.

Authors - Minbo Zhang
View Article