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验证数据展示

  • WB analysis of Jurkat using 25389-1-AP

    WB analysis of Jurkat using 25389-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25389-1-AP

    IHC staining of mouse brain using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25389-1-AP (CHERP Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 25389-1-AP

    WB analysis using 25389-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25389-1-AP

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 25389-1-AP

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHERP Polyclonal antibody

CHERP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25389-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CHERP, DAN16, DAN26, ERPROT 213 21, SCAF6, SRA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25389-1-AP targets CHERP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHERP fusion protein Ag13428 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium homeostasis endoplasmic reticulum protein
别名 CHERP, DAN16, DAN26, ERPROT 213 21, SCAF6, SRA1
计算分子量 104 kDa
观测分子量 130 kDa
GenBank蛋白编号BC021294
基因名称 CHERP
Gene ID (NCBI) 10523
RRIDAB_2880055
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHERP antibody 25389-1-APDownload protocol
IHC protocol for CHERP antibody 25389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols