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CHERP Polyclonal antibody

CHERP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25389-1-AP

Print datasheet

Synonyms

CHERP, DAN16, DAN26, ERPROT 213 21, SCAF6, SRA1

验证数据展示

  • WB analysis of Jurkat using 25389-1-AP

    WB analysis of Jurkat using 25389-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25389-1-AP

    IHC staining of mouse brain using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25389-1-AP (CHERP Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 25389-1-AP

    WB analysis using 25389-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25389-1-AP

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 25389-1-AP

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25389-1-AP targets CHERP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHERP fusion protein Ag13428 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium homeostasis endoplasmic reticulum protein
Synonyms CHERP, DAN16, DAN26, ERPROT 213 21, SCAF6, SRA1
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC021294
Gene Symbol CHERP
Gene ID (NCBI) 10523
RRIDAB_2880055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHERP antibody 25389-1-APDownload protocol
IHC protocol for CHERP antibody 25389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols