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验证数据展示

  • IHC staining of human lung cancer using 23342-1-AP

    IHC staining of human lung cancer using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human lung cancer using 23342-1-AP

    IHC staining of human lung cancer using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human small intestine using 23342-1-AP

    IHC staining of human small intestine using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human small intestine using 23342-1-AP

    IHC staining of human small intestine using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of mouse pancreas using 23342-1-AP

    IHC staining of mouse pancreas using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of mouse pancreas using 23342-1-AP

    IHC staining of mouse pancreas using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IF Staining of mouse small intestine using 23342-1-AP

    IF Staining of mouse small intestine using 23342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 23342-1-AP

    IF Staining of mouse small intestine using 23342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Neuro-2a using 23342-1-AP

    IF Staining of Neuro-2a using 23342-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Chromogranin A antibody (23342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 23342-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IF-P, ELISA and More (2)

别名

CHGA, CGA, ER-37, EA-92, Chromogranin-A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse pancreas tissue, human lung cancer tissue, human small intestine tissue, human colon tissue, mouse colon tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23342-1-AP targets Chromogranin A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Chromogranin A fusion protein Ag19780 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromogranin A (parathyroid secretory protein 1)
别名 CHGA, CGA, ER-37, EA-92, Chromogranin-A
计算分子量 51 kDa
GenBank蛋白编号BC006459
基因名称 Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2879259
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
IHC protocol for Chromogranin A antibody 23342-1-APDownload protocol
IF protocol for Chromogranin A antibody 23342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.

Authors - Buqing Ye
View Article
mouseIF

Cell Mol Gastroenterol Hepatol

Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia

Authors - Pawan Puri
View Article