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验证数据展示

  • WB analysis using 30419-1-AP

    WB analysis using 30419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30419-1-AP (CHI3L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 30419-1-AP

    IF Staining of THP-1 using 30419-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1/YKL40 antibody (30419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30419-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, HepG2 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30419-1-AP targets CHI3L1/YKL40 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CHI3L1/YKL40 fusion protein Ag33123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chitinase 3-like 1 (cartilage glycoprotein-39)
别名 CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39
计算分子量 383 aa, 43 kDa
观测分子量40-43 kDa
GenBank蛋白编号BC008568
基因名称 CHI3L1
Gene ID (NCBI) 1116
RRIDAB_3086309
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

实验方案

Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 30419-1-APDownload protocol
IF protocol for CHI3L1/YKL40 antibody 30419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols