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CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30419-1-AP

Print datasheet

Synonyms

CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39

验证数据展示

  • WB analysis using 30419-1-AP

    WB analysis using 30419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30419-1-AP (CHI3L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-29 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30419-1-AP targets CHI3L1/YKL40 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CHI3L1/YKL40 fusion protein Ag33123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Synonyms CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight40-43 kDa
GenBank Accession NumberBC008568
Gene Symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

实验方案

Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 30419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols