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验证数据展示

  • WB analysis of THP-1 using 83685-4-RR

    WB analysis of THP-1 using 83685-4-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83685-4-RR (CHI3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83685-4-RR

    IHC staining of human ovary cancer using 83685-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83685-4-RR (CHI3L1/YKL40 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83685-4-RR

    IHC staining of human ovary cancer using 83685-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83685-4-RR (CHI3L1/YKL40 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 83685-4-RR

    IF Staining of THP-1 using 83685-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1 antibody (83685-4-RR, Clone: 240691F11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of THP-1 using 83685-4-RR

    FC experiment of THP-1 using 83685-4-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug CHI3L1 Recombinant antibody (83685-4-RR, Clone:240691F11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83685-4-RR

    Affinity and Kinetic Characterization of 83685-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83685-4-RR against Human CHI3L1 were performed. The affinity constant is 3.14 nM.

CHI3L1/YKL40 Recombinant antibody

CHI3L1/YKL40 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83685-4-RR

产品说明书

CloneNo. 240691F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

CHI3L1, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39, CGP 39

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83685-4-RR targets CHI3L1/YKL40 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CHI3L1/YKL40 fusion protein Ag33123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chitinase 3-like 1 (cartilage glycoprotein-39)
别名 CHI3L1, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39, CGP 39
计算分子量 383 aa, 43 kDa
观测分子量40-43 kDa
GenBank蛋白编号BC008568
基因名称 CHI3L1
Gene ID (NCBI) 1116
RRIDAB_3671287
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

实验方案

Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
IHC protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
IF protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols