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CHIA Polyclonal antibody

CHIA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21484-1-AP

Print datasheet

Synonyms

TSA1902, Lung-specific protein TSA1902, Lung specific protein TSA1902, EC:3.2.1.14, AMCase

验证数据展示

  • WB analysis of mouse lung using 21484-1-AP

    WB analysis of mouse lung using 21484-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 21484-1-AP

    IHC staining of human stomach using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 21484-1-AP

    IHC staining of human stomach using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21484-1-AP

    IF Staining of HepG2 using 21484-1-AP

    Immunofluorescent analysis of HepG2 cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman stomach tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21484-1-AP targets CHIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CHIA fusion protein Ag15767 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chitinase, acidic
Synonyms TSA1902, Lung-specific protein TSA1902, Lung specific protein TSA1902, EC:3.2.1.14, AMCase
Calculated Molecular Weight 476 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC036339
Gene Symbol CHIA
Gene ID (NCBI) 27159
RRIDAB_10755301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZP6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHIA antibody 21484-1-APDownload protocol
IHC protocol for CHIA antibody 21484-1-APDownload protocol
IF protocol for CHIA antibody 21484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis.

Authors - Lei Zhao
View Article
mouseIF

Commun Biol

Elevated stress response marks deeply quiescent reserve cells of gastric chief cells

Authors - Daisuke Shiokawa
View Article
mouseWB,IF

Eur J Immunol

Aspergillus fumigatus corneal infection is regulated by chitin synthases and by neutrophil-derived acidic mammalian chitinase.

Authors - Steven de Jesus Carrion
View Article