Choline Kinase Alpha Recombinant antibody

Choline Kinase Alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84881-2-RR

产品说明书

CloneNo. 242095B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

CHKA, EC:2.7.1.32, CKI, CHK, CHETK-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84881-2-RR targets Choline Kinase Alpha in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Choline Kinase Alpha fusion protein Ag4761 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 choline kinase alpha
别名 CHKA, EC:2.7.1.32, CKI, CHK, CHETK-alpha
计算分子量 439 aa, 52 kDa
观测分子量 50 kDa
GenBank蛋白编号BC036471
基因名称 CHKA
Gene ID (NCBI) 1119
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Choline Kinase Alpha antibody 84881-2-RRDownload protocol
Standard Protocols
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