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CHMP4B Polyclonal antibody

CHMP4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 13683-1-AP

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Synonyms

SHAX1, hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B



经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue, HeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, C2C12 cells, H9C2 cells
Planning an IHC experiment? We recommend our IHCeasy CHMP4B Ready-To-Use IHC Kit. CHMP4B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13683-1-AP targets CHMP4B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Immunogen CHMP4B fusion protein Ag4544 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromatin modifying protein 4B
Synonyms SHAX1, hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight25-33 kDa
GenBank Accession NumberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
RRIDAB_2877971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

实验方案

Product Specific Protocols
WB protocol for CHMP4B antibody 13683-1-APDownload protocol
IHC protocol for CHMP4B antibody 13683-1-APDownload protocol
IF protocol for CHMP4B antibody 13683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanIF

Cell

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Authors - Suresh Kumar
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier

Neuron

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Authors - Juliet Goldsmith
humanCoIP

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
humanIF

Sci Adv

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Authors - Virginia Andrade