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验证数据展示

  • WB analysis using 68811-1-Ig

    WB analysis using 68811-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68811-1-Ig (CHMP4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HCT 116 using 68811-1-Ig

    IF Staining of HCT 116 using 68811-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using CHMP4B antibody (68811-1-Ig, Clone: 1D4G10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

CHMP4B Monoclonal antibody

CHMP4B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68811-1-Ig

产品说明书

CloneNo. 1D4G10

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
Positive IF/ICC detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy CHMP4B Ready-To-Use IHC Kit. CHMP4B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68811-1-Ig targets CHMP4B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 CHMP4B fusion protein Ag4544 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromatin modifying protein 4B
别名 C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2
计算分子量 224 aa, 25 kDa
观测分子量 33 kDa
GenBank蛋白编号BC033859
基因名称 CHMP4B
Gene ID (NCBI) 128866
RRIDAB_3670432
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H444
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

实验方案

Product Specific Protocols
WB protocol for CHMP4B antibody 68811-1-IgDownload protocol
IF protocol for CHMP4B antibody 68811-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols